A Co-Reactive Immunosensor Based on Ti 3 C 2 T x MXene@TiO 2 -MoS 2 Hybrids Promoting luminol@Au@Ni-Co NCs Electrochemiluminescence for CYFRA 21-1 Detection.

The construction of assays is capable of accurately detecting cytokeratin-19 (CYFRA 21-1), which is critical for the rapid diagnosis of nonsmall cell lung cancer. In this work, a novel electrochemiluminescence (ECL) immunosensor based on the co-reaction promotion of luminol@Au@Ni-Co nanocages (NCs) as ECL probe by Ti 3 C 2 T x MXene@TiO 2 -MoS 2 hybrids as co-reaction accelerator was proposed to detect CYFRA 21-1. Ni-Co NCs, as a derivative of Prussian blue analogs, can be loaded with large quantities of Au NPs, luminol, and CYFRA 21-1 secondary antibodies due to their high specific surface area. To further improve the sensitivity of the developed ECL immunosensor, Ti 3 C 2 T x MXene@TiO 2 -MoS 2 hybrids were prepared by in situ growth of TiO 2 nanosheets on highly conductive Ti 3 C 2 T x MXene, and MoS 2 was homogeneously grown on Ti 3 C 2 T x MXene@TiO 2 surfaces by the hydrothermal method. Ti 3 C 2 T x MXene@TiO 2 -MoS 2 hybrids possess excellent catalytic performance on the electro-redox of H 2 O 2 generating more O 2 ·- and obtaining optimal ECL intensity of the luminol/H 2 O 2 system. Under the appropriate experimental conditions, the quantitative detection range of CYFRA 21-1 was from 0.1 pg mL -1 to 100 ng mL -1 , and the limit of detection (LOD) was 0.046 pg mL -1 . The present sensor has a lower LOD with a wider linear range, which provides a new analytical assay for the early diagnosis of small-cell-type lung cancer labels.