Steric hindrance of N 6 -methyl in m 6 A and its application for specific loci detection.
Xiaoyi XieQi WeiJingyu HanXin FangWei YangXiang ZhouYafen WangXiaocheng WengPublished in: Chemical communications (Cambridge, England) (2024)
We found that the N 6 methyl group of N 6 -methyladenine is able to hinder the methylation of N 6 -methyladenine at the N 1 position by DMS. Based on this, we have devised a novel method for detecting N 6 -methyladenine, which was successfully applied to identify specific m 6 A loci in 28S rRNA.