Development of a Safe and Effective Bacillus thuringiensis -Based Nanobiopesticide for Controlling Tea Pests.

Mg(OH) 2 was used as the nanocarrier of the Bacillus thuringiensis (Bt) Cry1Ac protein, and the synthesized Cry1Ac-Mg(OH) 2 composites were regular and uniform nanosheets. Nano-Mg(OH) 2 could effectively improve the insecticidal effect of the Cry1Ac protein toward Ectropis obliqua . It could enhance the damage degree of the Cry1Ac protein to intestinal epithelial cells and microvilli, induce and enrich the production of reactive oxygen species (ROS) in the midgut, and enhance the degradation of the Cry1Ac protein into active fragments. Furthermore, an anti-rinsing assay showed that the Cry1Ac-Mg(OH) 2 composites were bound to the notch structure of the tea leaf surface. The retention of the Cry1Ac protein increased by 11.45%, and sprayed nano-Mg(OH) 2 was rapidly absorbed by different tissues of tea plants. Moreover, nano-Mg(OH) 2 and composites did not significantly affect non-target organisms. These results show that nano-Mg(OH) 2 can serve as a safe and effective biopesticide carrier, which provides a new approach for stable and efficient Bt preparation.