Serum- and xeno-free culture of human umbilical cord perivascular cells for pediatric heart valve tissue engineering.
Shouka Parvin NejadMonica LecceBahram MiraniNataly Machado SiqueiraZahra MirzaeiJ Paul SanterreJohn E DaviesCraig A SimmonsPublished in: Stem cell research & therapy (2023)
Overall, our findings establish an in vitro culture platform that uses hUCPVCs as a readily-available and non-invasively sourced autologous cell population and a commercial serum- and xeno-free culture medium to increase the translational potential of future pediatric HVTE strategies. This study evaluated the proliferative, differentiation and extracellular matrix (ECM) synthesis capacities of human umbilical cord perivascular cells (hUCPVCs) when cultured in serum- and xeno-free media (SFM) against conventionally used bone marrow-derived MSCs (BMMSCs) and serum-containing media (SCM). Our findings support the use of hUCPVCs and SFM for in vitro heart valve tissue engineering (HVTE) of autologous pediatric valve tissue. Figure created with BioRender.com.
Keyphrases
- umbilical cord
- mesenchymal stem cells
- tissue engineering
- extracellular matrix
- endothelial cells
- induced apoptosis
- cell therapy
- aortic valve
- mitral valve
- bone marrow
- aortic stenosis
- heart failure
- endoplasmic reticulum stress
- transcatheter aortic valve replacement
- coronary artery disease
- induced pluripotent stem cells
- single cell
- stem cells
- oxidative stress
- current status
- risk assessment
- atrial fibrillation
- high throughput
- ejection fraction
- childhood cancer