Design and Immunogenicity of SARS-CoV-2 DNA Vaccine Encoding RBD-PVXCP Fusion Protein.
Dmitri DormeshkinMikalai KatsinMaria StegantsevaSergey GolenchenkoMichail ShapiraSimon DubovikDzmitry LutskovichAnton KavaleuskiAlexander MeleshkoPublished in: Vaccines (2023)
The potential of immune-evasive mutation accumulation in the SARS-CoV-2 virus has led to its rapid spread, causing over 600 million confirmed cases and more than 6.5 million confirmed deaths. The huge demand for the rapid development and deployment of low-cost and effective vaccines against emerging variants has renewed interest in DNA vaccine technology. Here, we report the rapid generation and immunological evaluation of novel DNA vaccine candidates against the Wuhan-Hu-1 and Omicron variants based on the RBD protein fused with the Potato virus X coat protein (PVXCP). The delivery of DNA vaccines using electroporation in a two-dose regimen induced high-antibody titers and profound cellular responses in mice. The antibody titers induced against the Omicron variant of the vaccine were sufficient for effective protection against both Omicron and Wuhan-Hu-1 virus infections. The PVXCP protein in the vaccine construct shifted the immune response to the favorable Th1-like type and provided the oligomerization of RBD-PVXCP protein. Naked DNA delivery by needle-free injection allowed us to achieve antibody titers comparable with mRNA-LNP delivery in rabbits. These data identify the RBD-PVXCP DNA vaccine platform as a promising solution for robust and effective SARS-CoV-2 protection, supporting further translational study.
Keyphrases
- sars cov
- circulating tumor
- cell free
- single molecule
- low cost
- nucleic acid
- protein protein
- coronavirus disease
- copy number
- machine learning
- diabetic rats
- metabolic syndrome
- drug induced
- electronic health record
- small molecule
- type diabetes
- high throughput
- risk assessment
- ultrasound guided
- adipose tissue
- skeletal muscle
- loop mediated isothermal amplification
- single cell
- quantum dots