Investigating the potential of delignified rice husk as a carbon-rich resource for extracting glucose and its utilization in biocement production through fungal isolates.

Burning rice straw is now a significant issue faced by different regions in India, as its burning releases harmful gases, mainly carbon dioxide. Various techniques are now in trend to utilize the rice straw, e.g., producing compressed natural gas using rice straw, bioethanol, etc., as a substrate for various microorganisms. A high quantity of non-utilized rice husk generates more ideas for its proper utilization. The cellulose, hemicellulose, and lignin found in rice straws can be a fungi growth medium. In this research, the delignification of rice husk is done by acid (2% and 4% H 2 SO 4 ) and alkali (2% and 4% NaOH) at 121 °C at 103 kPa for 1 h to obtain crude carbon source which is further utilized for biomineralization. The glucose is subjected to qualitative and quantitative analysis using Molisch's and Dinitro salicylic tests. The delignification process showed a positive outcome when 2% H 2 SO 4 is utilized maximum yield of 5.9 ug/ml free sugar concentration. Representing the highest glucose yield compared to the experiment's other acid and base substances used. Various techniques such as field emission-scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), and Fourier transformed infra-red (FTIR) spectroscopy are employed to examine surface and chemical alterations. The 2% H 2 SO 4 pretreated rice husk is utilized for microbial-induced calcite precipitation using fungal isolates S1 (3), S1 (18), and S4 (1). The calcite and vaterite produced by biomineralization are confirmed using XRD for fungal isolates namely, S1 (3), S1 (18), and S4 (1) having percentage crystallinity of 59%, 46.428%, and 62.69% percentage crystallinity respectively.