Stimuli-responsive multifunctional glyconanoparticle platforms for targeted drug delivery and cancer cell imaging.
Xumeng WuYu Jia TanHui Ting TohLan Huong NguyenShu Hui KhoSing Yian ChewHo Sup YoonXue-Wei LiuPublished in: Chemical science (2017)
Targeted bioimaging or chemotherapeutic drug delivery to achieve the desired therapeutic effects while minimizing side effects has attracted considerable research attention and remains a clinical challenge. Presented herein is a multi-component delivery system based on carbohydrate-functionalized gold nanoparticles conjugated with a fluorophore or prodrug. The system leverages active targeting based on carbohydrate-lectin interactions and release of the payload by biological thiols. Cell-type specific delivery of the activatable fluorophore was examined by confocal imaging on HepG2 cells, and displays distinct selectivity towards HepG2 cells over HeLa and NIH3T3 cells. The system was further developed into a drug delivery vehicle with camptothecin (CPT) as a model drug. It was demonstrated that the complex exhibits similar cytotoxicity to that of free CPT towards HepG2 cells, and is significantly less cytotoxic to normal HDF and NIH3T3 cells, indicating excellent specificity. The delivery vehicle itself exhibits excellent biocompatibility and offers an attractive strategy for cell-type specific delivery depending on the carbohydrates conjugated in the system.
Keyphrases
- cancer therapy
- drug delivery
- fluorescent probe
- gold nanoparticles
- drug release
- high resolution
- living cells
- photodynamic therapy
- quantum dots
- fluorescence imaging
- mass spectrometry
- structural basis
- cell proliferation
- optical coherence tomography
- tandem mass spectrometry
- liquid chromatography
- tissue engineering
- cell cycle arrest