Recent advances in optical biosensing and imaging of telomerase activity and relevant signal amplification strategies.
Ruining YangJunbo HuLongsheng ZhangXingfen LiuYanqin HuangLingzhi WuQu-Li FanPublished in: The Analyst (2024)
Telomerase as a new valuable biomarker for early diagnosis and prognosis evaluation of cancer has attracted much interest in the field of biosensors, cell imaging, and drug screening. In this review, we mainly focus on different optical techniques and various signal amplification strategies for telomerase activity determination. Fluorometric, colorimetry, chemiluminescence, surface-enhanced Raman scattering (SERS), and dual-mode techniques for telomerase sensing and imaging are summarized. Signal amplification strategies include two categories: one is nucleic acid-based amplification, such as rolling circle amplification (RCA), the hybridization chain reaction (HCR), and catalytic hairpin assembly (CHA); the other is nanomaterial-assisted amplification, including metal nanoclusters, quantum dots, transition metal compounds, graphene oxide, and DNA nanomaterials. Challenges and prospects are also discussed to provide new insights for future development of multifunctional strategies and techniques for in situ and in vivo analysis of biomarkers for accurate cancer diagnosis.
Keyphrases
- nucleic acid
- high resolution
- label free
- sensitive detection
- quantum dots
- papillary thyroid
- transition metal
- gold nanoparticles
- molecularly imprinted
- mass spectrometry
- stem cells
- squamous cell carcinoma
- emergency department
- cancer therapy
- young adults
- bone marrow
- drug induced
- circulating tumor
- circulating tumor cells
- liquid chromatography
- metal organic framework
- cell free