Tween 80 Micelles Loaded with Fe 3 O 4 Nanoparticles and Artemisinin for Combined Oxygen-Independent Ferroptosis Therapy of Cancer.
Junming CuiXinxi CaiRui QianLin WuXueyong QiJin CaoSong ShenPublished in: Pharmaceutics (2024)
Artemisinin has an endoperoxide bridge structure, which can be cleaved by ferrous ions to generate various carbonyl radicals in an oxygen-independent manner, highlighting its potential for treating hypoxic tumors. In our study, we fabricated Tween 80 micelles loaded with Fe 3 O 4 nanoparticles and artemisinin for cancer therapy. The synthesized Fe 3 O 4 nanoparticles and drug-loaded micelles have particle sizes of about 5 nm and 80 nm, respectively, both exhibiting excellent dispersibility and stability. After uptake by MCF-7 cells, drug-loaded micelles release Fe 2+ and ART into the cytoplasm, effectively inducing the generation of reactive oxygen species (ROS) in hypoxic conditions, thereby enhancing toxicity against cancer cells. In vitro and in vivo studies have demonstrated that ART and Fe 3 O 4 nanoparticles are encapsulated in Tween 80 to form micelles, which effectively prevent premature release during circulation in the body. Although free ART and Fe 3 O 4 nanoparticles can inhibit tumor growth, TW80-Fe 3 O 4 -ART micelles demonstrate a more pronounced inhibitory effect, with a tumor suppression rate of up to 85%. A novel strategy based on artemisinin and ferroptosis is thus offered, holding a favorable prospect for hypoxic cancer therapy.
Keyphrases
- cancer therapy
- drug delivery
- reactive oxygen species
- drug release
- cell death
- hiv infected
- plasmodium falciparum
- photodynamic therapy
- squamous cell carcinoma
- papillary thyroid
- stem cells
- cell cycle arrest
- emergency department
- current status
- walled carbon nanotubes
- bone marrow
- endoplasmic reticulum stress
- smoking cessation
- lymph node metastasis
- breast cancer cells
- quantum dots