Manipulation of bicarbonate concentration in sperm capacitation media improvesin vitro fertilisation output in porcine species.

Optimising HCO3 - concentrations allows for establishing an IVF method that significantly reduced porcine polyspermy and increased the production of viable zygotes. A concentration of 15 mmol/L of HCO3 - in the medium is sufficient to trigger the in vitro sperm capacitation and increase the fertilisation efficiency in porcine.