pH-Responsive Oxygen and Hydrogen Peroxide Self-Supplying Nanosystem for Photodynamic and Chemodynamic Therapy of Wound Infection.
Yunsu MaHuanghuang XuBo SunSilin DuShuai CuiLi ZhangNing DingDongzhi YangPublished in: ACS applied materials & interfaces (2021)
The combination of photodynamic therapy (PDT) and chemodynamic therapy (CDT) has been continuously explored in the antibacterial aspect and has achieved more effective antibacterial effect than a single therapy. We design a pH-responsive O2 and H2O2 self-supplying zeolitic imidazolate framework-67 (ZIF-67) nanosystem for PDT/CDT of wound infection. Under the acidic inflammatory conditions, ZIF-67 can degrade to produce Co2+ and release CaO2 and graphene quantum dots (GQDs). The exposed CaO2 reacted with water to generate H2O2 and O2. The self-supplied O2 alleviates hypoxia at the site of inflammation and enhances external light-initiated GQD-mediated PDT, while H2O2 was catalyzed by endogenous Co2+ to produce hydroxyl radicals for Co2+-triggered CDT. In vitro and in vivo experiments confirm that CaO2/GQDs@ZIF-67 has a combined PDT/CDT effect. The antibacterial mechanism indicates that bacteria post-treated with CaO2/GQDs@ZIF-67 may be sterilized by reactive oxygen species-mediated oxidative stress and the leakage of bacterial contents. The experiments also find that CaO2/GQDs@ZIF-67 may activate the immune response and enhance the therapeutic effect by activating the cyclic GMP-AMP synthase-stimulator of interferon genes signaling pathway.
Keyphrases
- photodynamic therapy
- oxidative stress
- hydrogen peroxide
- signaling pathway
- immune response
- quantum dots
- fluorescence imaging
- reactive oxygen species
- nitric oxide
- dendritic cells
- wound healing
- silver nanoparticles
- induced apoptosis
- drug delivery
- genome wide
- endothelial cells
- epithelial mesenchymal transition
- pi k akt
- dna damage
- ischemia reperfusion injury
- anti inflammatory
- transcription factor
- mesenchymal stem cells
- dna methylation
- cell therapy
- bone marrow
- cell proliferation
- diabetic rats
- heat stress