Usefulness of an RNA extraction-free test for the multiplexed detection of ALK , ROS1 , and RET gene fusions in in real life FFPE specimens of non-small cell lung cancers.

Francesca DamiolaAlberti LaurentMansuet-Lupo AudreyDamotte DianeHofman VéroniqueTixier LuciePenault-Llorca FréderiqueRouquette IsabelleVignaud Jean MichelCazes AurelieForest FabienBegueret HuguesGibault LaureBadoual CécileCayre AnneTaranchon-Clermont EstelleDuc AdelineMc Leer AnneLantuejoul Sylvie

Published in: Expert review of molecular diagnostics (2023)

Overall, the HTG EdgeSeq assay offers comparable sensitivities and specificity than other RNA sequencing techniques, with the advantage that it can be used on very small and old samples collected multicentrically.

Keyphrases

single cell
high throughput
cell death
dna damage
genome wide
cell therapy
advanced non small cell lung cancer
copy number
reactive oxygen species
loop mediated isothermal amplification
label free
real time pcr
stem cells
oxidative stress
dna methylation
structural basis
transcription factor