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Multiplex peptide microarray profiling of antibody reactivity against neglected tropical diseases derived B-cell epitopes for serodiagnosis in Zimbabwe.

Arthur VengesaiThajasvarie NaickerHerald MidziMaritha KasambalaTariro L Mduluza-JokonyaSimbarashe RusakanikoFrancisca MutapiTakafira Mduluza
Published in: PloS one (2022)
Multiplex peptide microarrays are a valuable tool for integrated NTDs surveillance and for screening parasites exposure in endemic areas. Species sero-reactivity observed in the study maybe indicative of exposure to the different NTDs parasites. However, although peptides with the least cross reactivity were selected there is need to validate the sero-reactivity with recombinant antigens and immune-blotting techniques such as western blotting.
Keyphrases
  • high throughput
  • public health
  • plasmodium falciparum
  • south africa
  • real time pcr
  • climate change
  • dendritic cells
  • cell free
  • human immunodeficiency virus
  • hiv testing