A tolane-modified 5-ethynyluridine as a universal and fluorogenic photochemical DNA crosslinker.

Published in: Chemical communications (Cambridge, England) (2023)

We report the fluorescent nucleoside Tol dU and its application as a photoresponsive crosslinker in three different DNA architectures with enhanced fluorescence emission of the crosslinked products. The fluorogenic Tol dU crosslinking reaction enables the assembly of DNA polymers in a hybridization chain reaction for the concentration-dependent detection of a specific DNA sequence.

Keyphrases

single molecule
circulating tumor
cell free
nucleic acid
living cells
label free
quantum dots
hyaluronic acid
real time pcr
electron transfer
fluorescent probe