Enteric pathogenic bacteria and resistance gene carriage in the homeless population in Marseille, France.
Tran Duc Anh LyLinda HadjadjVan Thuan HoangNdiaw GoumbalaThi Loi DaoSekene BadiagaHerve Tissot-DupontPhilippe BrouquiDidier RaoultJean-Marc RolainPhilippe GautretPublished in: Acta microbiologica et immunologica Hungarica (2021)
We aimed to assess the prevalence of pathogenic bacteria and resistance genes in rectal samples collected among homeless persons in Marseille, France. In February 2014 we enrolled 114 sheltered homeless adults who completed questionnaires and had rectal samples collected. Eight types of enteric bacteria and 15 antibiotic resistance genes (ARGs) were sought by real-time polymerase chain reaction (qPCR) performed directly on rectal samples. ARG-positive samples were further tested by conventional PCR and sequencing. We evidenced a 17.5% prevalence of gastrointestinal symptoms, a 9.6% prevalence of enteric pathogenic bacteria carriage, including Escherichia coli pathotypes (8.7%) and Tropheryma whipplei (0.9%). Only 2 persons carried blaCTX-M-15 resistance genes (1.8%), while other genes, including carbapenemase-encoding genes and colistin-resistance genes, (mcr-1 to mcr-6, mcr-8) were not detected. Our results suggest that sheltered homeless persons in Marseille do not have a high risk of harbouring gastrointestinal antibiotic resistant bacteria.
Keyphrases
- escherichia coli
- klebsiella pneumoniae
- genome wide
- genome wide identification
- antibiotic resistance genes
- multidrug resistant
- bioinformatics analysis
- risk factors
- mental illness
- genome wide analysis
- rectal cancer
- microbial community
- acinetobacter baumannii
- dna methylation
- drug resistant
- gram negative
- pseudomonas aeruginosa
- copy number
- cystic fibrosis
- single cell
- mental health
- biofilm formation
- staphylococcus aureus
- gene expression
- depressive symptoms