Versatile fluorescence detection of microRNA based on novel DNA hydrogel-amplified signal probes coupled with DNA walker amplification.

Chunli LiHongkun LiJunjun GeGuifen Jie

Published in: Chemical communications (Cambridge, England) (2019)

A novel DNA hydrogel-amplified versatile fluorescence platform combined with hybridization chain reaction (HCR) and DNA walking multiple amplification was developed for ultrasensitive detection of miRNA. The DNA hydrogel was loaded with large amounts of SYBR Green (SG) I dyes or CdTe quantum dots (QDs) to assemble versatile signal probes.

Keyphrases

single molecule
nucleic acid
circulating tumor
quantum dots
drug delivery
cell free
label free
living cells
wound healing
small molecule
gold nanoparticles
energy transfer
loop mediated isothermal amplification
sensitive detection
fluorescence imaging
photodynamic therapy
high throughput