Targeted Therapy with Cisplatin-Loaded Calcium Citrate Nanoparticles Conjugated with Epidermal Growth Factor for Lung Cancer Treatment.
Lipika OopkaewYuwanda InjongkolNatchanon RimsuebPanupong MahalapbutrKiattawee ChoowongkomonSarinya HadsadeeRojrit RojanathanesThanyada RungrotmongkolPublished in: ACS omega (2024)
Lung cancer is the leading cause of cancer-related deaths worldwide with high incidence rates for new cases. Conventional cisplatin (CDDP) therapy has limitations due to severe side effects from nonspecific targeting. To address this challenge, nanomedicine offers targeted therapies. In this study, cisplatin-loaded calcium citrate nanoparticles conjugated with epidermal growth factor (CaCit@CDDP-EGF NPs) were synthesized. The resulting nanodrug had a size below 350 nm with a cation charge. Based on density functional theory (DFT), the CaCit@CDDP NP model containing two citrates substituted on two chlorides exhibited a favorable binding energy of -5.42 eV, and the calculated spectrum at 261 nm closely matched the experimental data. CaCit@CDDP-EGF NPs showed higher inhibition rates against EGFR-expressed and mutant carcinoma cells compared to those of cisplatin while displaying lower cytotoxicity to lung fibroblast cells. Integrating in vitro experiments with in silico studies, these nanoparticles hold promise as a novel nanomedicine for targeted therapy in clinical applications.
Keyphrases
- growth factor
- density functional theory
- cancer therapy
- photodynamic therapy
- wound healing
- drug delivery
- molecular docking
- molecular dynamics
- small cell lung cancer
- induced apoptosis
- big data
- risk factors
- early onset
- tyrosine kinase
- oxide nanoparticles
- cell cycle arrest
- machine learning
- oxidative stress
- walled carbon nanotubes
- bone marrow
- cell proliferation
- transcription factor
- dna binding
- deep learning