What can Ribo-seq and proteomics tell us about the non-canonical proteome?
John R PrensnerJennifer G AbelinLeron W KokKarl R ClauserJonathan M MudgeJorge Ruiz-OreraMichal Bassani-SternbergEric W DeutschSebastiaan van HeeschPublished in: bioRxiv : the preprint server for biology (2023)
Combined use of Ribo-seq and proteomics-based methods enables optimal confidence in detecting non-canonical ORFs and their protein products.Ribo-seq can provide more sensitive detection of non-canonical ORFs, but data quality and analytical pipelines will impact results.Non-canonical ORF catalogs are diverse and span both high-stringency and low-stringency ORF nominations.A framework for standardized non-canonical ORF evidence will advance the research field.