The chemistry of Vernonanthura nudiflora (Less.) H. Rob. flowers and its antimicrobial activities.
Anderson Valdiney Gomes RamosNathália de SáDébora Larissa Oliveira AraújoMárcia Regina Pereira CabralGiovana Ferreira CostacurtaBeatriz Cardoso de FreitasLenisa Vieira VilegasRegiane Bertin de Lima ScodroVera Lucia Dias SiqueiraErika Seki Kioshima CoticaMarta Regina Barrotto do CarmoMaria Helena SarragiottoDebora Cristina BaldoquiPublished in: Natural product research (2021)
Twenty-one known specialised metabolites were isolated from the flowers of Vernonanthura nudiflora (Less.) H. Rob., the structures of the compounds were established based on 1 D and 2 D NMR spectroscopic experiments. Others 28 compounds were putatively identified using the dereplication technique by UHPLC-HRMS/MS. Twenty-three of the compounds are being reported for the first time in this species. The mixture of sesquiterpene lactones piptocarphins A and B (17 + 18), and the flavone velutin (14) were tested against several microorganisms and showed promising activity against Mycobacterium tuberculosis with MIC of 15.6 μg/mL and 31.2 μg/mL, respectively. Furthermore, 17 + 18 showed greater cytotoxicity against VERO cells (IC50 = 7.0 ± 1.73) compared to compound 14 (IC50 85.0 ± 10.6 µg/mL). These findings reveal the feasibility of using the UHPLC-ESI-HRMS/MS-based dereplication strategy in complex fractions to identify specialised metabolites, moreover to V. nudiflora flowers being a source of compounds with antimycobacterial potential.
Keyphrases
- ms ms
- mycobacterium tuberculosis
- high resolution mass spectrometry
- induced apoptosis
- high resolution
- mass spectrometry
- magnetic resonance
- multiple sclerosis
- molecular docking
- ultra high performance liquid chromatography
- risk assessment
- genome wide
- cell cycle arrest
- gene expression
- cell death
- cell proliferation
- climate change
- endoplasmic reticulum stress
- dna methylation