Polypyrrole Nanocones and Dynamic Piezoelectric Stimulation-Induced Stem Cell Osteogenic Differentiation.
Zhengnan ZhouPeng YuLei ZhouLingjie TuLei FanFengmiao ZhangCong DaiYi LiuChengyun NingJianqiang DuGuoxin TanPublished in: ACS biomaterials science & engineering (2019)
Imitating the physiological microenvironment of living cell and tissues opens new avenues of research into the application of electricity to medical therapies. In this study, dynamic piezoelectric stimulation is generated in a dynamic culture because of the piezoelectric effect of the poly(vinylidene fluoride)-polypyrrole (PVDF-PPy) electroactive composite. Combined with PPy nanocones, dynamic piezoelectric signals are effectively and continuously provided to cells. In the presence of dynamic piezoelectric stimulation and PPy nanocones, PPy-PVDF NS samples show promoted bone mesenchymal stem cell (BMSCs) adhesion, spreadin, and osteogenic differentiation. On the basis of the results of this study, PPy nanocones and dynamic piezoelectric stimulation can be administered to modulate cell behavior, paving the way for the exploration of cellular responses to dynamic electrical stimulation.
Keyphrases
- mesenchymal stem cells
- stem cells
- bone marrow
- healthcare
- gene expression
- single cell
- cystic fibrosis
- escherichia coli
- staphylococcus aureus
- cell death
- cell proliferation
- induced apoptosis
- signaling pathway
- zika virus
- cell cycle arrest
- endothelial cells
- gold nanoparticles
- dengue virus
- umbilical cord
- drug induced
- soft tissue
- molecularly imprinted
- solid phase extraction
- simultaneous determination