Comparative Study of Nitro- and Azide-Functionalized Zn II -Based Coordination Polymers (CPs) as Fluorescent Turn-On Probes for Rapid and Selective Detection of H 2 S in Living Cells.
Subhajit SahaPritam Kumar RoyKartik MaityMahitosh MandalKumar BiradhaPublished in: Chemistry (Weinheim an der Bergstrasse, Germany) (2022)
Selective detection of H 2 S in the cellular systems using fluorescent CPs/MOFs is of great scientific interest due to their outstanding aqueous stability, biocompatibility and real-time detection ability. Fabrication of such materials using complete biologically essential elements and applying them as an efficient biosensor is still quite challenging. In this context, two newly synthesized CPs containing biologically essential metal ion (Zn) and nitro/azido functional groups into the framework to sense extracellular and intracellular H 2 S by reducing into respective amines are presented. The CP-1 containing the azide group acted as an efficient fluorescent turn-on probe with the lowest detection limit (7.2 μM) and shortest response time (30 s) among the Zn-based probes reported till date. Moreover, CP-1 exhibited green luminescence in live cells after imaging a very low concentration of H 2 S, whereas the nitro analogue CP-2 could not detect the target analyte due to its framework disruption.
Keyphrases
- living cells
- fluorescent probe
- loop mediated isothermal amplification
- label free
- quantum dots
- single molecule
- sensitive detection
- real time pcr
- heavy metals
- induced apoptosis
- risk assessment
- cell death
- signaling pathway
- gold nanoparticles
- oxidative stress
- cell cycle arrest
- ionic liquid
- cell proliferation
- metal organic framework
- tissue engineering
- energy transfer