Polyphenols, antioxidants, and wound healing of Lecythis pisonis seed coats.

To better use the Lecythis pisonis Cambess. biomass, this study investigates whether Sapucaia seed coats present wound-healing properties. We analyzed the antibacterial, antioxidant, and wound healing-promoting potentials, plus cytotoxicity and stimulation of VEGF-A. The chemical composition was analyzed by positive ion-mode electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI(+)FT-ICR MS). A total of 19 compounds were identified, such as proanthocyanidin A1, procyanidin A1, B2, C1, epigallocatechin, kaempferol (p-coumaroyl) glycoside, and kaempferol (p-coumaroyl) glycoside. Potent antioxidant strength/index was verified for DPPH (IC50 0.99 µg/mL) and FRAP (IC50 1.09 µg/mL). The extract did not present cytotoxicity and promoted significant cell migration and/or proliferation of fibroblasts (p<0.05). VEGF-A was stimulated dose-dependently, 6 µg/mL (167.13±8.30 pg/mL), 12.5 µg/mL (210.3±14.2 pg/mL) and 25 µg/mL (411.6±29.4 pg/mL), PDGF (0.002 µg/mL) stimulated 215.98 pg/mL. Staphylococcus aureus was susceptible to the extract, with a minimum inhibitory concentration (MIC) of 31.25 µg/mL. The identified compounds benefit the antioxidant activity, promoting hemostasis for the wound healing process, indicating that this extract has the potential for use in dermatological cosmetics. Keywords: Lecythis pisonis Cambess., Lecythidaceae, chemical analysis, wound healing. Abbreviations: DPPH (2,2-diphenyl-1-picrylhydrazyl radical), ESI(+)FT-ICR MS (Positive-ion mode electrospray ionization with Fourier transform ion cyclotron resonance mass spectrometry), FRAP (Ferric Reducing Antioxidant Power), GAE (Gallic acid equivalent), MIC (minimum inhibitory concentration), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, VEGF-A (vascular endothelial growth factor). .