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Enhanced low-cost lipopeptide biosurfactant production by Bacillus velezensis from residual glycerin.

Henrique A BritoAmanda P NappEvandro PereiraEvelise BachJoão V B BorowskiLuciane M P PassagliaVania M M MeloRaphael MoreiraE Johan FosterFernanda C LopesMarilene H Vainstein
Published in: Bioprocess and biosystems engineering (2024)
Biosurfactants (BSFs) are molecules produced by microorganisms from various carbon sources, with applications in bioremediation and petroleum recovery. However, the production cost limits large-scale applications. This study optimized BSFs production by Bacillus velezensis (strain MO13) using residual glycerin as a substrate. The spherical quadratic central composite design (CCD) model was used to standardize carbon source concentration (30 g/L), temperature (34 °C), pH (7.2), stirring (239 rpm), and aeration (0.775 vvm) in a 5-L bioreactor. Maximum BSFs production reached 1527.6 mg/L of surfactins and 176.88 mg/L of iturins, a threefold increase through optimization. Microbial development, substrate consumption, concentration of BSFs, and surface tension were also evaluated on the bioprocess dynamics. Mass spectrometry Q-TOF-MS identified five surfactin and two iturin isoforms produced by B. velezensis MO13. This study demonstrates significant progress on BSF production using industrial waste as a microbial substrate, surpassing reported concentrations in the literature.
Keyphrases
  • mass spectrometry
  • low cost
  • systematic review
  • heavy metals
  • bacillus subtilis
  • high resolution
  • capillary electrophoresis
  • simultaneous determination