The use of commercial fibrin glue in dermal replacement material reduces angiogenic and lymphangiogenic gene and protein expression in vitro.
Benedikt FuchsAlexandra BirtNicholas MoellhoffConstanze KuhlmannRiccardo GiuntaPaul Severin WiggenhauserPublished in: Journal of biomaterials applications (2023)
Our findings demonstrate the impact of fibrin glue application in dermal regeneration with special regard to angiogenesis and lymphangiogenesis. In particular, a short fibrin treatment of 24 hours led to a decrease in gene and protein levels of LECS, HUVECs, and ASCs. In contrast, the long-term application showed less effect on gene and protein expressions. Therefore, this work demonstrated the negative effects of fibrin-treated cells in tissue engineering approaches and could affect wound healing during dermal regeneration.
Keyphrases
- wound healing
- tissue engineering
- copy number
- genome wide
- stem cells
- platelet rich plasma
- genome wide identification
- induced apoptosis
- magnetic resonance
- magnetic resonance imaging
- binding protein
- computed tomography
- cell cycle arrest
- protein protein
- contrast enhanced
- transcription factor
- oxidative stress
- gene expression
- smoking cessation
- vascular endothelial growth factor
- cell proliferation