Sequencing analysis and efficient biodiesel production by lipase from Pseudomonas aeruginosa.
Israa M S Al KadmySarah Naji AzizNadheema Hammood HusseinSamah N El-ShafeiyIsraa Hussein HamzahAhmed SuhailEman AlhomaidiAbdelazeem M AlgammalGaber El-Saber BatihaHala M ElBadreHelal F HettaPublished in: Molecular biology reports (2024)
Four P. aeruginosa isolates were obtained from different environmental sources (soil), phenotypically identified, and it was confirmed by the PCR detection of the 16SrRNA gene. The isolated P. aeruginosa strains were screened for lipase production, and the recovered lipase was purified. Besides, the lipase (lip) gene was detected by PCR, and the purified PCR products were sequenced and analyzed. The production of biofuel was conducted using gas chromatography among tested oils. It was found that castor oil was the best one that enhances lipase production in-vitro.