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An ESIPT-induced NIR fluorescent probe to visualize mitochondrial sulfur dioxide during oxidative stress in vivo.

Haixian RenFangjun HuoXia WuXiaogang LiuCaixia Yin
Published in: Chemical communications (Cambridge, England) (2021)
Based on the change in electron distribution of the benzopyrylium unit before and after sulfite addition, a 2-(2'-hydroxyphenyl)benzothiazole (HBT)-based fluorophore generated the excited state intramolecular proton transfer (ESIPT) process with a near-infrared enhanced emission at 836 nm and a large Stokes shift (286 nm). The probe was applied to image SO2 derivatives in cells and mice. Our data will provide new ideas for the development of ESIPT-based fluorescence probe with longer wavelength emission.
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