Development a multiplex panel of AISNPs, multi-allelic InDels, microhaplotypes and Y-SNP/InDel loci for multiple forensic purposes via the NGS.

Recently, next generation sequencing showed the promising application value in forensic research. In this study, we constructed a multiplex system of different molecular genetic markers based on the previous selected AISNPs, multi-allelic InDels, microhaplotypes and Y-SNP/InDel loci and evaluated forensic efficiencies of the system in Chinese Shaanxi Han, Hui and Mongolian groups via the NGS platform. Ancestry informative analyses of Shaanxi Han, Hui and Mongolian groups revealed that most Mongolian individuals could be differentiated from Shaanxi Hans and Huis based on the selected AISNPs. Multi-allelic InDels and microhaplotypes showed the multiple allele variations and possessed relatively high genetic polymorphisms in these three groups, indicating these loci could also provide higher forensic efficiencies for individual identification and paternity testing. Based on Y-SNPs, different haplogroup distributions were observed among Shaanxi Han, Hui and Mongolian groups. In conclusion, the self-developed system could be used to simultaneously carry out the individual identification, paternity analysis, mixture deconvolution, forensic ancestry information analysis and Y chromosomal haplogroup inference, which could provide more investigative clues in forensic practices. This article is protected by copyright. All rights reserved.