Sensitive detection methods are key to identify secondary EGFR c.2369C>T p.(Thr790Met) in non-small cell lung cancer tissue samples.

Cleo KeppensElisabeth M C DequekerEtienne RouleauNils 't HartLukas BubendorfKelly DufraingCéline GarrecPaul GuéguenAude LamyAntonio MarchettiPatrick PauwelsAles RyskaVéronique TackLuigi TornilloKaat Van CasterenJan H von der ThüsenKaren ZwaenepoelBirgit Lissenberg-WitteErik ThunnissenEd Schuuring

Published in: BMC cancer (2020)

A significant number of laboratories that offer p.(Thr790Met) testing did not detect this relevant mutation compared to the other EQA participants. However, correct identification of this variant is improving over time and was higher for NGS users. Revising the methodology might be useful to resolve errors, especially for resection specimens with low frequency or multiple variants. EQA providers should include challenging resections in the scheme.

Keyphrases

sensitive detection
tyrosine kinase
epidermal growth factor receptor
quantum dots
small cell lung cancer
copy number
loop mediated isothermal amplification
patient safety
liver metastases
adverse drug
emergency department