Positive Effects of Oral Antibiotic Administration in Murine Chronic Graft-Versus-Host Disease.
Shinri SatoEisuke ShimizuJingliang HeMamoru OgawaKazuki AsaiHiroyuki YazuRobert RuschMio YamaneFan YangShinji FukudaYutaka KawakamiMasayuki OhtaYoko OgawaPublished in: International journal of molecular sciences (2021)
Chronic graft-versus-host disease (cGVHD) is one of the most frequent complications experienced after allogeneic hematopoietic stem cell transplantation. Reportedly, dysbiosis and severe damage to the microbiome are also closely associated with GVHD. Herein, we aimed to elucidate the positive and negative effects of the administration of various antibiotics in a murine model of cGVHD. For allogeneic bone marrow transplantation (allo-BMT), bone marrow from B10.D2 mice were transplanted in BALB/c mice to induce cGVHD. The cGVHD mice were orally administered ampicillin, gentamicin (GM), fradiomycin, vancomycin, or the solvent vehicle (control group). Among the antibiotic-treated mice, the systemic cGVHD phenotypes and ocular cGVHD manifestations were suppressed significantly in GM-treated mice compared to that in control mice. Inflammatory cell infiltration and fibrosis in cGVHD-targeted organs were significantly attenuated in GM-treated mice. Although regulatory T cells were retained at greater levels in GM-treated mice, there were significantly fewer Th17 cells and interleukin (IL)-6-producing macrophages in cGVHD-targeted organs in these mice. Collectively, our results revealed that orally administered GM may exert positive effects in a cGVHD mouse model.
Keyphrases
- high fat diet induced
- bone marrow
- regulatory t cells
- allogeneic hematopoietic stem cell transplantation
- mouse model
- acute lymphoblastic leukemia
- oxidative stress
- single cell
- metabolic syndrome
- wild type
- adipose tissue
- signaling pathway
- staphylococcus aureus
- stem cells
- insulin resistance
- acute myeloid leukemia
- risk factors
- immune response
- methicillin resistant staphylococcus aureus