Aquaporin-4 Autoantibody Detection by ELISA: A Retrospective Characterization of a Commonly Used Assay.
Jon P WilliamsJustin R AbbatemarcoJonathan J GalliStefanie J RodenbeckLisa K PetersonThomas R HavenMeagan StreetJohn W RoseJohn E GreenleeM Mateo Paz SoldanStacey L ClardyPublished in: Multiple sclerosis international (2021)
Our data illustrates diagnostic uncertainty associated with the AQP4 ELISA, an assay that is still commonly ordered by clinicians despite the availability of more sensitive and specific tests to detect AQP4 autoantibodies in patients suspected of having NMOSD. In particular, low positive titer AQP4 ELISA results are particularly nonspecific for the diagnosis of NMOSD. The importance of accessibility to both sensitive and specific AQP4 testing cannot be overemphasized in clinical practice.