Real-Time Fluorescence Screening Platform for Meat Freshness.
Huan YeSeyoung Koonull Beitong ZhuYingjun KeRuilong ShengTing DuanLintao ZengJong Seung KimPublished in: Analytical chemistry (2022)
Meat's freshness is closely related to food safety and human health and has received increasing attention nowadays. To on-site visually screen meat freshness in a fast and non-destructive manner, we rationally constructed a series of fluorescent probes ( JDCN , JDNS , and JDPY ) with distinct electron-withdrawing substitution groups based on julolidine-fused coumarin. These probes underwent an aza-Michael addition followed by an elimination reaction with cadaverine to generate a colorimetric and ratiometric fluorescence response, and their sensing performance was rationally enhanced by improving the electron-withdrawing strength of substitution groups. Particularly, JDCN with a dicyanovinyl group as the reaction site exhibited outstanding sensing performance including rapid response (∼60 s), high selectivity, and low detection limit (14 nM). Furthermore, JDCN was fabricated into test kits to detect cadaverine vapor with a high-contrast fluorescence change from red to green. Based on two-color visualization of cadaverine vapor, on-site non-contact and non-destructive monitoring of meat freshness was successfully achieved. The good sensing performance rendered JDCN test kits a promising real-time fluorescence screening platform for rapid, non-destructive, and accurate evaluation of meat freshness.
Keyphrases
- single molecule
- living cells
- human health
- fluorescent probe
- energy transfer
- loop mediated isothermal amplification
- risk assessment
- high throughput
- quantum dots
- sensitive detection
- small molecule
- gold nanoparticles
- magnetic resonance
- climate change
- high resolution
- hydrogen peroxide
- fluorescence imaging
- magnetic resonance imaging
- electron microscopy
- label free
- working memory
- electron transfer
- computed tomography
- nucleic acid
- light emitting