Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer.

Megumi MiyanoKosei TanakaShu IshikawaShinji TakenakaAndrés Miguel-ArribasWilfried J J MeijerKen-Ichi Yoshida

Published in: Microbial cell factories (2018)

The chromosomal DNA mobilization mediated by pLS20catΔoriT will allow us to develop a novel genetic tool for the rapid, easy, and repetitive mobilization of longer DNA segments into a recipient chromosome.

Keyphrases

circulating tumor
copy number
cell free
bacillus subtilis
single molecule
escherichia coli
crispr cas
genome wide
high frequency
regulatory t cells
dendritic cells
immune response
dna methylation
anaerobic digestion