Physiological and transcriptomic response to methyl-coenzyme M reductase limitation in Methanosarcina acetivorans .

cells grown in substrate-replicate batch cultures produce more MCR than its cellular demand for optimal growth. The tools outlined in this study can be used to refine metabolic models of methanogenesis and assay lesions in MCR in a higher-throughput manner than isolation and biochemical characterization of pure protein.