Simultaneous detection of JAK2, CALR, and MPL mutations and quantitation of JAK2 V617F allele burden in myeloproliferative neoplasms using the quenching probe-Tm method in i-densy IS-5320.
Kunihito AraiMasahiro SakaguchiShunsuke YuiTomoaki KitanoMiho MiyataMayumi YogosawaKazutaka NakayamaKenji TajikaKensuke UsukiJunya KurodaNobuhiko UoshimaYutaka KobayashiHitoji UchiyamaYasushi KubotaShinya KimuraShinichiro MoriMitsuharu HiraiSatoshi WakitaHiroki YamaguchiPublished in: International journal of laboratory hematology (2022)
Overall, i-densy IS-5320 exhibited good accuracy in terms of analyzing MPN-associated genes; thus, it can serve as a replacement for conventional methods of MPN-associated gene testing.
Keyphrases
- genome wide identification
- genome wide
- ms ms
- mass spectrometry
- genome wide analysis
- copy number
- liquid chromatography tandem mass spectrometry
- quantum dots
- living cells
- loop mediated isothermal amplification
- dna methylation
- liquid chromatography
- label free
- real time pcr
- high performance liquid chromatography
- risk factors
- tandem mass spectrometry
- energy transfer
- solid phase extraction
- fluorescent probe