Metastatic Colorectal Cancer Treatment Response Evaluation by Ultra-Deep Sequencing of Cell-Free DNA and Matched White Blood Cells.

Iris van 't Erve Jamie E Medina Alessandro Leal Eniko Papp Jillian A Phallen Vilmos Adleff Elaine Jiayuee Chiao Adith S Arun Karen Bolhuis John K Simmons Aanavi Karandikar Kenneth C Valkenburg Mark Sausen Samuel V Angiuoli Robert B Scharpf Cornelis J A Punt Gerrit A Meijer Victor E Velculescu Remond J A Fijneman

Published in: Clinical cancer research : an official journal of the American Association for Cancer Research (2023)

Accurate calling of ctDNA mutations for treatment response monitoring is feasible in a tumor tissue-independent manner by combined cfDNA and patient-matched WBC genomic DNA analysis. This tissue biopsy-independent approach simplifies sample logistics and facilitates the application of liquid biopsy ctDNA testing for evaluation of emerging therapy resistance, opening new avenues for early adaptation of treatment regimens.

Keyphrases

circulating tumor
metastatic colorectal cancer
induced apoptosis
ultrasound guided
high resolution
cell free
fine needle aspiration
cell cycle arrest
case report
stem cells
copy number
cell death
signaling pathway
endoplasmic reticulum stress
mass spectrometry
mesenchymal stem cells