A 3D Biocompatible Plasmonic Tweezer for Single Cell Manipulation.
Siyu KangMuhammad Shemyal NisarYu LuNing ChangYan HuangHaibin NiSergey M NovikovYi WangQiannan CuiXiang-Wei ZhaoPublished in: Small methods (2023)
Plasmonic tweezers are an emerging research topic because of their low input power and wide operating range from homogeneous particles to complex biological objects. But it is still challenging for plasmonic tweezers to trap or manipulate objects of tens of microns, especially in biological science. This study introduces a new 3D biocompatible plasmonic tweezer for single living cell manipulation in solution. The key design is a tapered tip whose three-layer surface structure consists of nanoprobe, gold nanofilm, and thermosensitive hydrogel, thiolated poly(N-isopropylacrylamide). Incident light excites the surface plasmon polaritons on gold film and generates heat to induce thermally driven phase transition of the thermosensitive hydrogel, which enables reversible binding between functionalized surface and cell membrane and avoids both thermal and mechanical stresses in the meanwhile. The 3D biocompatible plasmonic tweezer realizes selective capture, 3D pathway free transport, and position-controlled release of target cells, and it displays excellent biocompatibility, low energy consumption, and high operational flexibility.
Keyphrases
- single molecule
- energy transfer
- single cell
- label free
- ionic liquid
- induced apoptosis
- public health
- cardiovascular disease
- hyaluronic acid
- cell cycle arrest
- oxidative stress
- type diabetes
- high throughput
- stem cells
- living cells
- reduced graphene oxide
- signaling pathway
- cell proliferation
- transcription factor
- mesenchymal stem cells
- binding protein
- endoplasmic reticulum stress
- dna binding