Proteome profile of the cerebellum from α7 nicotinic acetylcholine receptor deficient mice.
Karolina Magdalena CabanPia SeßenhausenJan Bernard StöcklBastian PopperArtur MayerhoferFröhlich ThomasPublished in: Proteomics (2024)
The alpha7 nicotinic acetylcholine receptor (α7 nAChR; CHRNA7) is expressed in the nervous system and in non-neuronal tissues. Within the central nervous system, it is involved in various cognitive and sensory processes such as learning, attention, and memory. It is also expressed in the cerebellum, where its roles are; however, not as well understood as in the other brain regions. To investigate the consequences of absence of CHRNA7 on the cerebellum proteome, we performed a quantitative nano-LC-MS/MS analysis of samples from CHRNA7 knockout (KO) mice and corresponding wild type (WT) controls. Liver, an organ which does not express this receptor, was analyzed, in comparison. While the liver proteome remained relatively unaltered (three proteins more abundant in KOs), 90 more and 20 less abundant proteins were detected in the cerebellum proteome of the KO mice. The gene ontology analysis of the differentially abundant proteins indicates that the absence of CHRNA7 leads to alterations in the glutamatergic system and myelin sheath in the cerebellum. In conclusion, our dataset provides new insights in the role of CHRNA7 in the cerebellum, which may serve as a basis for future in depth-investigations.
Keyphrases
- wild type
- working memory
- white matter
- gene expression
- high fat diet induced
- high resolution
- type diabetes
- binding protein
- optical coherence tomography
- multiple sclerosis
- resting state
- skeletal muscle
- genome wide
- current status
- copy number
- mass spectrometry
- transcription factor
- metabolic syndrome
- functional connectivity