Detection of Mpox Virus Using Microbial Cell-free DNA: the Potential of Pathogen-Agnostic Sequencing for Rapid Identification of Emerging Pathogens.
Sarah Y ParkMartin S LindnerKevin BrickNicholas NollRachid OunitLuis J NoaRabeeya SabzwariRonald TribleJason C SniffenPrerana RothAmir KhanAnamaria RodriguezSyeda SahraMichael J DavisInderjeet S BrarGayathri BalasundaramFrederick S NolteTimothy A BlauwkampBradley A PerkinsSivan BercoviciPublished in: The Journal of infectious diseases (2023)
We demonstrate the potential of plasma mcfDNA sequencing to detect, quantify, and, for acute infections with high sequencing coverage, subtype MPXV using a single non-invasive test. Sequencing plasma mcfDNA may augment existing mpox testing in vulnerable patient populations or in patients with atypical symptoms or unrecognized mpox. Strain type information may supplement disease surveillance and facilitate tracking emerging pathogens.
Keyphrases
- single cell
- public health
- gram negative
- liver failure
- healthcare
- case report
- antimicrobial resistance
- human health
- health information
- depressive symptoms
- drug induced
- climate change
- extracorporeal membrane oxygenation
- hepatitis b virus
- acute respiratory distress syndrome
- genetic diversity
- sensitive detection
- disease virus