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A hybridization-triggered DNAzyme cascade assay for enzyme-free amplified fluorescence detection of nucleic acids.

Huizhen WangDinggeng HeRi WuHong ChengWenjie MaJin HuangHongchang BuXiaoxiao HeKemin Wang
Published in: The Analyst (2019)
An enzyme-free and ultrasensitive fluorescence assay for the detection of nucleic acids was successfully established by a hybridization-triggered DNAzyme cascade (HTDC). This simple and cost-effective sensor has good sensitivity, selectivity and the capability for detection of target DNA from complex fluids.
Keyphrases
  • label free
  • single molecule
  • loop mediated isothermal amplification
  • living cells
  • real time pcr
  • high throughput
  • quantum dots
  • nucleic acid