A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq.

Published in: PeerJ (2016)

This method could be scaled up to generate many thousands of sequences per MiSeq run and could be applied to other sequencing platforms. This has great potential for populating databases with high quality, near full-length 16S rRNA gene sequences from under-represented taxa and environments and facilitates analyses of microbial communities at higher resolution.

Keyphrases

genome wide
single cell
genome wide identification
copy number
genome wide analysis
dna methylation
single molecule
big data
genetic diversity
transcription factor
bioinformatics analysis
machine learning
high throughput sequencing