Integrated Smartphone-App-Chip System for On-Site Parts-Per-Billion-Level Colorimetric Quantitation of Aflatoxins.
Xiaochun LiFan YangJessica X H WongHua-Zhong YuPublished in: Analytical chemistry (2017)
We demonstrate herein an integrated, smartphone-app-chip (SPAC) system for on-site quantitation of food toxins, as demonstrated with aflatoxin B1 (AFB1), at parts-per-billion (ppb) level in food products. The detection is based on an indirect competitive immunoassay fabricated on a transparent plastic chip with the assistance of a microfluidic channel plate. A 3D-printed optical accessory attached to a smartphone is adapted to align the assay chip and to provide uniform illumination for imaging, with which high-quality images of the assay chip are captured by the smartphone camera and directly processed using a custom-developed Android app. The performance of this smartphone-based detection system was tested using both spiked and moldy corn samples; consistent results with conventional enzyme-linked immunosorbent assay (ELISA) kits were obtained. The achieved detection limit (3 ± 1 ppb, equivalent to μg/kg) and dynamic response range (0.5-250 ppb) meet the requested testing standards set by authorities in China and North America. We envision that the integrated SPAC system promises to be a simple and accurate method of food toxin quantitation, bringing much benefit for rapid on-site screening.
Keyphrases
- high throughput
- circulating tumor cells
- loop mediated isothermal amplification
- label free
- ms ms
- high resolution
- mass spectrometry
- single cell
- sensitive detection
- liquid chromatography tandem mass spectrometry
- real time pcr
- liquid chromatography
- high performance liquid chromatography
- escherichia coli
- tandem mass spectrometry
- human health
- gold nanoparticles
- deep learning
- risk assessment
- nitric oxide
- simultaneous determination
- fluorescent probe
- machine learning
- fluorescence imaging
- photodynamic therapy