The impact of zwitterionic surfactants on optode-based nanosensors via different fabrication approaches and sensing mechanisms.

In this work, we explored the impact of zwitterionic surfactants, sulfobetaine 16 (SB-16) and a PEG-phospholipid conjugate (DSPE-PEG), on nanosensor performance. We fabricated four sensors (for Na + , K + , Al 3+ , and O 2 ) and examined how these surfactants influenced various aspects, including fabrication methods, sensing mechanisms, and the incorporation of nanomaterials. Our results highlighted SB-16's role in enhancing selectivity in ion-exchange sensors (Na + and K + ) while maintaining sensitivity akin to its PEG counterpart. The liquid-liquid extraction based sensors (Al 3+ ) were unaffected by surfactant choice, while the O 2 sensors that operate via collisional quenching exhibited reduced sensitivity with SB-16 when compared to its PEG-based counterpart. Additionally, the SB-16 sensors proved adaptable to different fabrication approaches (SESE - single emulsion solvent evaporation and FNP - flash nanoprecipitation), showcased good cell viability and maintained a functional lifetime of at least five days. Furthermore, via the use of quantum dots, we showed that it is possible to incorporate other nanomaterials into the sensing phase of SB-16 sensors. Future investigations could target enhancing the pH stability of zwitterionic surfactants to further advance their applicability in sensor technologies.