Multiplex Lithographic SERS Aptasensor for Detection of Several Respiratory Viruses in One Pot.
Vladimir I KukushkinOganes AmbartsumyanAlexei SubekinAnna AstrakhantsevaVladimir A GushchinAlexandra NikonovaAnastasia DorofeevaVitaly ZverevAnna KeshekNadezda MeshcheryakovaOlga ZaborovaAlexandra GambaryanElena G ZavyalovaPublished in: International journal of molecular sciences (2023)
Rapid and reliable techniques for virus identification are required in light of recurring epidemics and pandemics throughout the world. Several techniques have been distributed for testing the flow of patients. Polymerase chain reaction with reverse transcription is a reliable and sensitive, though not rapid, tool. The antibody-based strip is a rapid, though not reliable, and sensitive tool. A set of alternative tools is being developed to meet all the needs of the customer. Surface-enhanced Raman spectroscopy (SERS) provides the possibility of single molecule detection taking several minutes. Here, a multiplex lithographic SERS aptasensor was developed aiming at the detection of several respiratory viruses in one pot within 17 min. The four labeled aptamers were anchored onto the metal surface of four SERS zones; the caught viruses affect the SERS signals of the labels, providing changes in the analytical signals. The sensor was able to decode mixes of SARS-CoV-2 (severe acute respiratory syndrome coronavirus two), influenza A virus, respiratory syncytial virus, and adenovirus within a single experiment through a one-stage recognition process.
Keyphrases
- loop mediated isothermal amplification
- sensitive detection
- raman spectroscopy
- label free
- sars cov
- respiratory syndrome coronavirus
- single molecule
- quantum dots
- real time pcr
- respiratory syncytial virus
- gold nanoparticles
- end stage renal disease
- chronic kidney disease
- high throughput
- ejection fraction
- newly diagnosed
- coronavirus disease
- transcription factor
- patient reported outcomes
- respiratory tract
- peritoneal dialysis
- prognostic factors
- positron emission tomography
- mass spectrometry
- fluorescent probe