Friunavirus Phage-Encoded Depolymerases Specific to Different Capsular Types of Acinetobacter baumannii .
Olga Yu TimoshinaAnastasia A KasimovaMikhail M ShneiderIlya O MatyutaAlena Y NikolaevaPeter V EvseevNikolay P ArbatskyAlexander S ShashkovAlexander O ChizhovAndrey A ShelenkovYulia V MikhaylovaPavel V SlukinNikolay V VolozhantsevKonstantin M BoykoYuriy A KnirelKonstantin A MiroshnikovAnastasiya V PopovaPublished in: International journal of molecular sciences (2023)
Acinetobacter baumannii is a critical priority nosocomial pathogen that produces a variety of capsular polysaccharides (CPSs), the primary receptors for specific depolymerase-carrying phages. In this study, the tailspike depolymerases (TSDs) encoded in genomes of six novel Friunaviruses, APK09, APK14, APK16, APK86, APK127v, APK128, and one previously described Friunavirus phage, APK37.1, were characterized. For all TSDs, the mechanism of specific cleavage of corresponding A. baumannii capsular polysaccharides (CPSs) was established. The structures of oligosaccharide fragments derived from K9, K14, K16, K37/K3-v1, K86, K127, and K128 CPSs degradation by the recombinant depolymerases have been determined. The crystal structures of three of the studied TSDs were obtained. A significant reduction in mortality of Galleria mellonella larvae infected with A. baumannii of K9 capsular type was shown in the example of recombinant TSD APK09_gp48. The data obtained will provide a better understanding of the interaction of phage-bacterial host systems and will contribute to the formation of principles of rational usage of lytic phages and phage-derived enzymes as antibacterial agents.
Keyphrases
- acinetobacter baumannii
- pseudomonas aeruginosa
- multidrug resistant
- drug resistant
- cystic fibrosis
- cardiovascular disease
- cardiovascular events
- machine learning
- big data
- dna binding
- candida albicans
- cell free
- type diabetes
- artificial intelligence
- silver nanoparticles
- drosophila melanogaster
- water soluble
- mass spectrometry
- methicillin resistant staphylococcus aureus