Trimanganese Tetroxide Nanozyme protects Cartilage against Degeneration by Reducing Oxidative Stress in Osteoarthritis.
Wenhan WangJiazhi DuanWenjun MaBowei XiaFeng LiuYing KongBoyan LiHang ZhaoLiang WangKeyi LiYiwei LiXiheng LuZhichao FengYuanhua SangGang LiHao XueJichuan QiuHong LiuPublished in: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2023)
Osteoarthritis, a chronic degenerative cartilage disease, is the leading cause of movement disorders among humans. Although the specific pathogenesis and associated mechanisms remain unclear, oxidative stress-induced metabolic imbalance in chondrocytes plays a crucial role in the occurrence and development of osteoarthritis. In this study, a trimanganese tetroxide (Mn 3 O 4 ) nanozyme with superoxide dismutase (SOD)-like and catalase (CAT)-like activities is designed to reduce oxidative stress-induced damage and its therapeutic effect is investigated. In vitro, Mn 3 O 4 nanozymes are confirmed to reprogram both the imbalance of metabolism in chondrocytes and the uncontrolled inflammatory response stimulated by hydrogen peroxide. In vivo, a cross-linked chondroitin sulfate (CS) hydrogel is designed as a substrate for Mn 3 O 4 nanozymes to treat osteoarthritis in mouse models. As a result, even in the early stage of OA (4 weeks), the therapeutic effect of the Mn 3 O 4 @CS hydrogel is observed in both cartilage metabolism and inflammation. Moreover, the Mn 3 O 4 @CS hydrogel maintained its therapeutic effects for at least 7 days, thus revealing a broad scope for future clinical applications. In conclusion, these results suggest that the Mn 3 O 4 @CS hydrogel is a potentially effective therapeutic treatment for osteoarthritis, and a novel therapeutic strategy for osteoarthritis based on nanozymes is proposed.
Keyphrases
- hydrogen peroxide
- knee osteoarthritis
- oxidative stress
- rheumatoid arthritis
- drug delivery
- early stage
- hyaluronic acid
- room temperature
- inflammatory response
- transition metal
- metal organic framework
- extracellular matrix
- tissue engineering
- nitric oxide
- wound healing
- risk assessment
- mouse model
- ischemia reperfusion injury
- dna damage
- high resolution
- lymph node
- induced apoptosis
- single molecule
- current status
- preterm birth
- amino acid
- atomic force microscopy