Chemical Characterization and Immunomodulatory Activity of Fucoidan from Sargassum hemiphyllum .
Rui LiQing-Ling ZhouShu-Tong ChenMin-Rui TaiHong-Ying CaiRui DingXiao-Fei LiuJian-Ping ChenLian-Xiang LuoSai-Yi ZhongPublished in: Marine drugs (2022)
Fucoidan is a sulfated algal polyanionic polysaccharide that possesses many biological activities. In this paper, a fucoidan (SHF) polysaccharide was extracted from Sargassum hemiphyllum collected in the South China Sea. The SHF, with a molecular weight of 1166.48 kDa (44.06%, w / w ), consisted of glucose (32.68%, w / w ), galactose (24.81%, w / w ), fucose (20.75%, w / w ), xylose (6.98%, w / w ), mannose (2.76%, w / w ), other neutral monosaccharides, and three uronic acids, including glucuronic acid (5.39%, w / w ), mannuronic acid (1.76%, w / w ), and guronuronic acid (1.76%, w / w ). The SHF exhibited excellent immunostimulatory activity. An immunostimulating assay showed that SHF could significantly increase NO secretion in macrophage RAW 264.7 cells via upregulation of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) levels based on both gene expression and protein abundance. These results suggest that SHF isolated from Sargassum hemiphyllum has great potential to act as a health-boosting ingredient in the pharmaceutical and functional-food fields.
Keyphrases
- nitric oxide synthase
- gene expression
- nitric oxide
- induced apoptosis
- healthcare
- public health
- dna methylation
- adipose tissue
- cell cycle arrest
- cell proliferation
- high throughput
- signaling pathway
- blood pressure
- oxidative stress
- risk assessment
- cell death
- skeletal muscle
- heat shock protein
- protein protein
- social media
- weight loss
- antibiotic resistance genes
- microbial community
- single cell
- insulin resistance
- amino acid
- glycemic control