Exploring the Molecular Tapestry: Organ-Specific Peptide and Protein Ultrafiltrates and Their Role in Therapeutics.
Jakub Peter SlivkaChris BauerAlexander YounsiMichelle B F WongMike K S ChanThomas SkutellaPublished in: International journal of molecular sciences (2024)
This study aims to characterize the proteome composition of organ-derived protein extracts from rabbits. Protein isolation was performed using soft homogenization and size exclusion via ultrafiltration. The proteome analysis of the ultrafiltrates was conducted using gel electrophoresis, and the mass spectrometry data were subjected to gene ontology analysis. Proteomic profiling revealed comprehensive protein profiles associated with RNA regulation, fatty acid binding, inflammatory response, oxidative stress, and metabolism. Additionally, our results demonstrate the presence of abundant small proteins, as observed in the mass spectrometry datasets. Small proteins and peptides are crucial in transcription modulation and various biological processes. The protein networks identified in the ultrafiltrates have the potential to enhance and complement biological therapeutic interventions. Data are available via ProteomeXchange with identifier PXD050039.
Keyphrases
- mass spectrometry
- inflammatory response
- oxidative stress
- amino acid
- protein protein
- binding protein
- electronic health record
- liquid chromatography
- small molecule
- physical activity
- gene expression
- climate change
- transcription factor
- risk assessment
- dna methylation
- copy number
- machine learning
- high performance liquid chromatography
- hyaluronic acid
- data analysis