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Versatility of a Salmonella Loop-Mediated Isothermal Amplification Assay Using Multiple Platforms and Master Mixes in Animal Food Matrices.

Kelly J DomesleShenia R YoungRyan C McDonaldBeilei Ge
Published in: Journal of AOAC International (2022)
We demonstrated the LAMP assay's versatility on two platforms and three master mixes for the rapid and reliable screening of Salmonella in seven animal food matrixes. GspSSD2.0 was the fastest master mix (time to positive results as early as 3.5 min) while Genie II had several attractive features from a user perspective.
Keyphrases
  • loop mediated isothermal amplification
  • escherichia coli
  • sensitive detection
  • high throughput
  • listeria monocytogenes
  • human health
  • climate change