Highly Bright Gold Nanowires Arrays for Sensitive Detection of Urea and Urease.
Yan LiAowei ZhaoJieqiong WangJieyu YuFei XiaoHongcheng SunPublished in: Nanomaterials (Basel, Switzerland) (2022)
In this work, highly fluorescent gold nanowire arrays (Au NWs) are successfully synthesized by assembling Zn 2+ ions and non-emissive oligomeric gold-thiolate clusters using mercaptopropionic acid both as a reducing agent and a growth ligand. The synthesized Au NWs exhibited strong bluish green fluorescence with an absolute quantum yield up to 32% and possessed ultrasensitive pH stimuli-responsive performance in the range of 7.0-7.8. Based on the excellent properties of the as-prepared nanowire arrays, we developed a facile, sensitive, and selective fluorescent method for quantitative detection of urea and urease. The fabricated nanoprobe showed superior biosensing response characteristics with good linearities in the range of 0-100 μM for urea concentration and 0-12 U/L for urease activity. In addition, this fluorescent probe afforded relatively high sensitivity with the detection limit as low as 2.1 μM and 0.13 U/L for urea and urease, respectively. Urea in human urine and urease in human serum were detected with satisfied results, exhibiting a promising potential for biomedical application.
Keyphrases
- sensitive detection
- quantum dots
- loop mediated isothermal amplification
- living cells
- fluorescent probe
- label free
- energy transfer
- room temperature
- reduced graphene oxide
- single molecule
- high density
- endothelial cells
- high resolution
- heavy metals
- real time pcr
- molecular dynamics
- silver nanoparticles
- gold nanoparticles
- risk assessment
- cancer therapy
- light emitting