Simultaneous determination of four cobalamins in rat plasma using online solid phase extraction coupled to high performance liquid chromatography-tandem mass spectrometry: Application to pentylenetetrazole-induced seizures in Sprague-Dawley rats.
Xiaomei JiangYan WangJia LiuPublished in: PloS one (2022)
A high throughput and high sensitive online solid phase extraction coupled to high performance liquid chromatography-tandem mass spectrometry method was established and validated for simultaneous quantification of four cobalamins including OHCbl, CNCbl, AdoCbl and MeCbl in rat plasma. Analytes were first enriched on an online SPE Shim-pack MAYI-ODS column and then separated using a Poroshell 120 EC C18 analytical column. The linear range was from 0.05 to 5 ng/ml and the LLOQ was 0.05 ng/ml for all four cobalamins. In addition, this method was applied to determine the cobalamins levels in pentylenetetrazole-induced kindling rat model for the first time.
Keyphrases
- solid phase extraction
- liquid chromatography tandem mass spectrometry
- simultaneous determination
- high performance liquid chromatography
- liquid chromatography
- molecularly imprinted
- tandem mass spectrometry
- gas chromatography mass spectrometry
- ultra high performance liquid chromatography
- high throughput
- high glucose
- diabetic rats
- social media
- mass spectrometry
- gas chromatography
- health information
- drug induced
- high resolution mass spectrometry
- endothelial cells
- ms ms
- oxidative stress
- healthcare
- stress induced